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pcr kit  (TaKaRa)


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    Structured Review

    TaKaRa pcr kit
    Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 26614 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rt+pcr+kit/PrimeScript+RT+Master+Mix/pmc13011060-433-18-21
    Average 99 stars, based on 26614 article reviews
    pcr kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Expressing:

    Article Title: Identification of Genes Associated with the Pan-Vibrios Resistance (PVR) Trait of Pacific White Shrimp ( Litopenaeus vannamei ) Using a Genome-Wide Association Study.
    Article Snippet: Total RNA was extracted using an RNA Easy Fast Tissue/Cell Kit (TIANGEN, Beijing, China) and was reverse-transcribed into cDNA using a Prime ScriptTMII 1st Strand cDNA Synthesis Kit (Takara, Tokyo, Japan). .. The expression levels of the gene in the brain, eye stalk, hemocytes, gill, hepatopancreas, heart, muscle, intestine and stomach were detected by qRT-PCR using a RT-PCR kit (TaKaRa, Tokyo, Japan) and the β-actin gene was used as the reference gene (Table S1). qRT-PCR was performed using Thermal Cycler Dice®Real Time System III (TaKaRa, Tokyo, Japan) under the following conditions: 95 ◦C for 30 s, 40 cycles of 95 ◦C for 5 s and 56 ◦C for 30 s, reading of templates at 95 ◦C for 15 s, and a final melting curve from 60 to 95 ◦C. ..

    Article Title: Identification of Genes Associated with the Pan-Vibrios Resistance (PVR) Trait of Pacific White Shrimp ( Litopenaeus vannamei ) Using a Genome-Wide Association Study
    Article Snippet: Total RNA was extracted using an RNA Easy Fast Tissue/Cell Kit (TIANGEN, Beijing, China) and was reverse-transcribed into cDNA using a Prime ScriptTMII 1st Strand cDNA Synthesis Kit (Takara, Tokyo, Japan). .. The expression levels of the gene in the brain, eye stalk, hemocytes, gill, hepatopancreas, heart, muscle, intestine and stomach were detected by qRT-PCR using a RT-PCR kit (TaKaRa, Tokyo, Japan) and the β-actin gene was used as the reference gene ( ). qRT-PCR was performed using Thermal Cycler Dice ® Real Time System III (TaKaRa, Tokyo, Japan) under the following conditions: 95 °C for 30 s, 40 cycles of 95 °C for 5 s and 56 °C for 30 s, reading of templates at 95 °C for 15 s, and a final melting curve from 60 to 95 °C. ..

    Quantitative RT-PCR:

    Article Title: Identification of Genes Associated with the Pan-Vibrios Resistance (PVR) Trait of Pacific White Shrimp ( Litopenaeus vannamei ) Using a Genome-Wide Association Study.
    Article Snippet: Total RNA was extracted using an RNA Easy Fast Tissue/Cell Kit (TIANGEN, Beijing, China) and was reverse-transcribed into cDNA using a Prime ScriptTMII 1st Strand cDNA Synthesis Kit (Takara, Tokyo, Japan). .. The expression levels of the gene in the brain, eye stalk, hemocytes, gill, hepatopancreas, heart, muscle, intestine and stomach were detected by qRT-PCR using a RT-PCR kit (TaKaRa, Tokyo, Japan) and the β-actin gene was used as the reference gene (Table S1). qRT-PCR was performed using Thermal Cycler Dice®Real Time System III (TaKaRa, Tokyo, Japan) under the following conditions: 95 ◦C for 30 s, 40 cycles of 95 ◦C for 5 s and 56 ◦C for 30 s, reading of templates at 95 ◦C for 15 s, and a final melting curve from 60 to 95 ◦C. ..

    Article Title: Identification of Genes Associated with the Pan-Vibrios Resistance (PVR) Trait of Pacific White Shrimp ( Litopenaeus vannamei ) Using a Genome-Wide Association Study
    Article Snippet: Total RNA was extracted using an RNA Easy Fast Tissue/Cell Kit (TIANGEN, Beijing, China) and was reverse-transcribed into cDNA using a Prime ScriptTMII 1st Strand cDNA Synthesis Kit (Takara, Tokyo, Japan). .. The expression levels of the gene in the brain, eye stalk, hemocytes, gill, hepatopancreas, heart, muscle, intestine and stomach were detected by qRT-PCR using a RT-PCR kit (TaKaRa, Tokyo, Japan) and the β-actin gene was used as the reference gene ( ). qRT-PCR was performed using Thermal Cycler Dice ® Real Time System III (TaKaRa, Tokyo, Japan) under the following conditions: 95 °C for 30 s, 40 cycles of 95 °C for 5 s and 56 °C for 30 s, reading of templates at 95 °C for 15 s, and a final melting curve from 60 to 95 °C. ..

    Article Title: Identification and functional characterization of a novel gene conferring root rot resistance in Panax Notoginseng.
    Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS values were used as the threshold for candidate region selection. qRT-PCR assay Total RNA was extracted from P. notoginseng and tobacco using the methods described above, and cDNA was synthesized with the RT-PCR kit (Takara, Dalian, China). .. The relative expression levels of genes were measured using the ABI QuantStudio 5 Flex real-time PCR system (Applied Biosystems, USA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Identification of Genes Associated with the Pan-Vibrios Resistance (PVR) Trait of Pacific White Shrimp ( Litopenaeus vannamei ) Using a Genome-Wide Association Study.
    Article Snippet: Total RNA was extracted using an RNA Easy Fast Tissue/Cell Kit (TIANGEN, Beijing, China) and was reverse-transcribed into cDNA using a Prime ScriptTMII 1st Strand cDNA Synthesis Kit (Takara, Tokyo, Japan). .. The expression levels of the gene in the brain, eye stalk, hemocytes, gill, hepatopancreas, heart, muscle, intestine and stomach were detected by qRT-PCR using a RT-PCR kit (TaKaRa, Tokyo, Japan) and the β-actin gene was used as the reference gene (Table S1). qRT-PCR was performed using Thermal Cycler Dice®Real Time System III (TaKaRa, Tokyo, Japan) under the following conditions: 95 ◦C for 30 s, 40 cycles of 95 ◦C for 5 s and 56 ◦C for 30 s, reading of templates at 95 ◦C for 15 s, and a final melting curve from 60 to 95 ◦C. ..

    Article Title: Identification of Genes Associated with the Pan-Vibrios Resistance (PVR) Trait of Pacific White Shrimp ( Litopenaeus vannamei ) Using a Genome-Wide Association Study
    Article Snippet: Total RNA was extracted using an RNA Easy Fast Tissue/Cell Kit (TIANGEN, Beijing, China) and was reverse-transcribed into cDNA using a Prime ScriptTMII 1st Strand cDNA Synthesis Kit (Takara, Tokyo, Japan). .. The expression levels of the gene in the brain, eye stalk, hemocytes, gill, hepatopancreas, heart, muscle, intestine and stomach were detected by qRT-PCR using a RT-PCR kit (TaKaRa, Tokyo, Japan) and the β-actin gene was used as the reference gene ( ). qRT-PCR was performed using Thermal Cycler Dice ® Real Time System III (TaKaRa, Tokyo, Japan) under the following conditions: 95 °C for 30 s, 40 cycles of 95 °C for 5 s and 56 °C for 30 s, reading of templates at 95 °C for 15 s, and a final melting curve from 60 to 95 °C. ..

    Article Title: Bidirectional Association between Atrial Fibrillation and Ovarian Cancer: Evidence from the UK Biobank and Mendelian Randomization
    Article Snippet: According to the manufacturer’s protocol, total cellular RNA was extracted using TRIzol (Invitrogen) and stored at −80°C. .. For gene detection, cDNA was generated using the RT-PCR kit (Takara Bio, Inc.), following the manufacturer’s instructions. ..

    Article Title: miR-19a-3p and miR-19b-3p repress Nurr1 and Nur77 to promote microglial inflammation after spinal cord injury
    Article Snippet: After incubating the microglia cells for 24 h, we used the SV Total RNA Isolation kit (Promega, #Z3100) to lyse and isolate total RNA. .. The Advantage for RT-PCR kit (Clontech Laboratories, Takara Bio Company, #639506) was used to generate cDNA from 2 μg of microglia total RNA using oligo 12–18 primers in a final volume of 100 μL. .. For real time PCR, the LightCycler-Faststart DNA Master SYBR Green I kit (Roche Molecular Biochemicals) was utilized.

    Article Title: TCR-NF-κB signaling activates the SENP1-NR4A1 axis to fine-tune effector activation of CD8 + T cells.
    Article Snippet: Total RNA was extracted from cells using TRIzol reagent (Life Technologies #15596018). .. 1 μg of total RNA was reverse transcribed into complementary DNA using RT-PCR kit (Takara #RR036A) according to instruction manual. .. Real-time PCR was performed on the LC480 system (Roche) using TB Green Premix Ex Taq (Takara #RR420A).

    Article Title: Identification and functional characterization of a novel gene conferring root rot resistance in Panax Notoginseng.
    Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS values were used as the threshold for candidate region selection. qRT-PCR assay Total RNA was extracted from P. notoginseng and tobacco using the methods described above, and cDNA was synthesized with the RT-PCR kit (Takara, Dalian, China). .. The relative expression levels of genes were measured using the ABI QuantStudio 5 Flex real-time PCR system (Applied Biosystems, USA).

    Article Title: TMEM207-mediated the impairment of skin regeneration through YAP sequestration in an allergic contact dermatitis model
    Article Snippet: .. Synthesis of cDNA from total RNA and subsequent PCR was performed using an RT-PCR kit (TaKaRa), as previously described. ..

    Article Title: TMEM207-mediated the impairment of skin regeneration through YAP sequestration in an allergic contact dermatitis model
    Article Snippet: .. Synthesis of cDNA from total RNA and subsequent PCR was performed using an RT-PCR kit (TaKaRa), as previously described. ..

    Generated:

    Article Title: Bidirectional Association between Atrial Fibrillation and Ovarian Cancer: Evidence from the UK Biobank and Mendelian Randomization
    Article Snippet: According to the manufacturer’s protocol, total cellular RNA was extracted using TRIzol (Invitrogen) and stored at −80°C. .. For gene detection, cDNA was generated using the RT-PCR kit (Takara Bio, Inc.), following the manufacturer’s instructions. ..

    Reverse Transcription:

    Article Title: TCR-NF-κB signaling activates the SENP1-NR4A1 axis to fine-tune effector activation of CD8 + T cells.
    Article Snippet: Total RNA was extracted from cells using TRIzol reagent (Life Technologies #15596018). .. 1 μg of total RNA was reverse transcribed into complementary DNA using RT-PCR kit (Takara #RR036A) according to instruction manual. .. Real-time PCR was performed on the LC480 system (Roche) using TB Green Premix Ex Taq (Takara #RR420A).

    Selection:

    Article Title: Identification and functional characterization of a novel gene conferring root rot resistance in Panax Notoginseng.
    Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS values were used as the threshold for candidate region selection. qRT-PCR assay Total RNA was extracted from P. notoginseng and tobacco using the methods described above, and cDNA was synthesized with the RT-PCR kit (Takara, Dalian, China). .. The relative expression levels of genes were measured using the ABI QuantStudio 5 Flex real-time PCR system (Applied Biosystems, USA).

    Synthesized:

    Article Title: Identification and functional characterization of a novel gene conferring root rot resistance in Panax Notoginseng.
    Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS values were used as the threshold for candidate region selection. qRT-PCR assay Total RNA was extracted from P. notoginseng and tobacco using the methods described above, and cDNA was synthesized with the RT-PCR kit (Takara, Dalian, China). .. The relative expression levels of genes were measured using the ABI QuantStudio 5 Flex real-time PCR system (Applied Biosystems, USA).

    Polymerase Chain Reaction:

    Article Title: TMEM207-mediated the impairment of skin regeneration through YAP sequestration in an allergic contact dermatitis model
    Article Snippet: .. Synthesis of cDNA from total RNA and subsequent PCR was performed using an RT-PCR kit (TaKaRa), as previously described. ..

    Article Title: TMEM207-mediated the impairment of skin regeneration through YAP sequestration in an allergic contact dermatitis model
    Article Snippet: .. Synthesis of cDNA from total RNA and subsequent PCR was performed using an RT-PCR kit (TaKaRa), as previously described. ..



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